A new species of “cydippid” ctenophore, Pukia ohtsukai sp. nov., is described from material collected in Nishino-omote Port, Tanegashima Island, Japan. It can be distinguished from the only other member of the genus by the presence of perradial canals, lack of bilateral diverticula beneath the comb plates, a lower number of comb plates per comb row (19–21), the presence of black pigment patches at the oral ends of the comb rows and around the statocyst, and by the length of the pole plate, which extends only to the aboral tips of the comb rows.