Yonezawa, S., Nakano, T., Nakahama, N., Tomikawa, K. and Isagi, Y. (2020). Environmental DNA reveals cryptic diversity within the subterranean amphipod genus Pseudocrangonyx
Akatsuka & Komai, 1922 (Amphipoda: Crangonyctoidea: Pseudocrangonyctidae) from central Japan. Journal of Crustacean Biology. 40(4), 479–483.
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Yonezawa, S., Nakano, T., Nakahama, N., Tomikawa, K. and Isagi, Y
2020
Environmental DNA reveals cryptic diversity within the subterranean amphipod genus Pseudocrangonyx
Akatsuka & Komai, 1922 (Amphipoda: Crangonyctoidea: Pseudocrangonyctidae) from central Japan
Journal of Crustacean Biology
40(4), 479–483
Publication
Available for editors
An environmental DNA (eDNA) detection method confirms the presence of the subterranean
amphipod genus Pseudocrangonyx Akatsuka & Komai, 1922 in the city of Kyoto, Honshu,
Japan for the first time in more than 90 years. Phylogenetic trees using partial sequences of
the 16S rRNA gene of eDNA samples from Kyoto and specimens collected from various
locations in Japan and Korea highlight that two genetically distinct taxonomic units inhabit
Kyoto’s subterranean environments. Results reveal that the ranges of distinct phylogroups of
Pseudocrangonyx widely overlap with each other around Lake Biwa in central western Honshu.
The eDNA results increase our understanding of the species richness and complicated evolutionary
history of these stygobitic amphipods.