Sponges (Phylum Porifera) collected during annual multispecies surveys and opportunistic sampling in the Gulf of St. Lawrence were examined to produce an identification guide that includes images of external form, spicules, and skeletal arrangement. First developed in 2020 and updated here with three new species and revised imagery, this guide presents information and occurrence maps for 49 taxa. The guide includes catch codes specific to Fisheries and Oceans Canada surveys operating in both the southern and northern Gulf, but serves as a broader identification resource for the region. Some taxa remain uncertain and warrant further taxonomic review, but the guide is intended to assist research teams in more reliably identifying sponges during future surveys and improving the quality of species occurrence data in the region.