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Castillo-Páez, A.; Llera-Herrera, R.; Cruz-Barraza, J.A. (2021). De novo transcriptome assembly for two color types of the marine sponge Mycale (Carmia) cecilia. Molecular Biology Reports. online first.
404828
10.1007/s11033-021-06274-4 [view]
Castillo-Páez, A.; Llera-Herrera, R.; Cruz-Barraza, J.A.
2021
De novo transcriptome assembly for two color types of the marine sponge <em>Mycale (Carmia) cecilia</em>
Molecular Biology Reports
online first
Publication
Available for editors  PDF available [request]
Despite the ecological importance and the potential pharmacological application of the sponge Mycale (Carmia) cecilia, it is uncertain whether the body-color variation, even in individuals coexisting in the same area, is due to intraspecific phenotypic plasticity or corresponds to taxonomic divergence. This uncertainty is relatively common in several Porifera groups, which lack the resolution of morphological diagnostic characters and slow-evolving mitochondrial genomes as occurs in early splitting lineages. We sequenced the RNA of six individuals with two different body-color (green-morphotype and red-morphotype) collected at the same time side by side. High-throughput sequencing of cDNA libraries produced?~?129 million reads with a length of 150 bp. Each morphotype was assembled separately owing to the low overlapping in the global assembly. Metatranscriptome de novo assembly of the trimmed and normalized reads produced 461 thousand transcripts for the green-morphotype and 342 thousand for the red-morphotype (respectively). Over 30% of the transcripts contained Open Reading Frames (ORFs) with functional significance. BUSCO analysis of the ORFs of putative poriferan origin (31.3% green or 30.4% red) indicated that our assemblies are 60% complete. This is the first attempt to evaluate the morphological diversity in the species M. (C.) cecilia and the phylum Porifera at the transcriptomic level. Due to the minimum overlap of the assembly and that, the red-morphotype diverged significantly from the green-morphotype (original color of M. (C.) cecilia). Therefore, we suggest that the red-morphotype should undergo a complete taxonomic investigation and its taxonomic status be reviewed. We expect that the transcriptome assembly metrics can be useful for comparing other transcriptome assemblies of non-model organisms.
East Pacific
Molecular systematics, Molecular biology
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2021-04-19 09:35:08Z
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2021-04-20 07:30:31Z
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